The purpose of this package is to measure the effects of eutrophication on the natural environment, i.e. in conditions which are not affected by confinement (enclosure effect) which imposes restrictions on mesocosm experiments. Also, field sampling will allow the collection of information for areas with different environmental characteristics (type of sediment, water renewal rates, sources of enrichment etc) and their benthic communities and populations. The specific objectives of WP2 are: (a) the detection, by means of remote sensing techniques, of areas of historical high values of chlorophyll in the water column during the summer months and relatively low renewal rate of water masses, (b) organizing of field sampling of all biotic communities to be studied in the water column and the sediment, of physical parameters (CTD casts for temperature, salinity, transparency, etc.) of geochemical variables in the column (pheopigments, chlorophyll, POC, PON, DOC, O2) and the sediment (Redox, pH, TOC, TON, TP, sulfides), estimation of sedimentation with sediment traps and conducting in situ experiments to determine the fluxes of N, P, at the sediment/water interface. Sampling should be done in the period after the spring peak of phytoplankton and before the establishment of strong temperature stratification.
-The study of historical data using remote sensing techniques of coastal areas of the Aegean and Ionian Seas showing high concentrations of chlorophyll and the identification of the maximum values in space and time. The monitoring process will continue with real-time-data and during the sampling mission and the group in charge of the sampling mission will be updated on an ongoing basis.
-The selection of the most suitable areas for sampling and organization of the expedition.
-The completion of the expedition using the HCMR research vessel F/R PHILIAin these identified areas. Measurement of parameters, field experiments, sample collection and preservation. Sorting in the field of organizations needed for genetic analysis.
-After completion of the analysis (WP4), drafting of scientific publications based on the results of the expedition.